ABSTRACT
RESUMEN La panoftalmitis es una forma rara y grave ocular de presentación del melanoma coroideo, por lo que, ante la presencia de una masa intraocular e inflamación de todas las estructuras intraoculares, con extensión extraescleral y los tejidos vecinos de la órbita, debe considerarse el diagnóstico diferencial de esta neoplasia maligna. Se presenta el caso de un paciente masculino de 83 años de edad, con diagnóstico de panoftalmitis como presentación atípica de un melanoma coroideo. Acudió a consulta de Cuerpo de Guardia de Oftalmología con dolor ocular intenso en ojo derecho, acompañado de náuseas y vómitos. Se constató, al examen, hipertensión ocular, que evolucionó tórpidamente durante su ingreso, con inflamación intraocular con extensión extraescleral. Se le realizaron ultrasonido ocular y tomografía axial computarizada de órbitas y se diagnosticó una panoftalmitis secundaria a un melanoma coroideo. Los hallazgos clínicos de esta forma atípica de presentación se mostraron, cuyo tratamiento definitivo es la enucleación. El diagnóstico histológico de melanoma maligno coroideo necrótico fue confirmado.
ABSTRACT Panophthalmitis is a rare and severe ocular form of presentation of choroid melanoma, therefore, in the presence of intraocular mass and inflammation of all intraocular structures, with extra-scleral extension and to the tissues neighboring the orbit, the differential diagnosis of this malignant neoplasia should be considered. The case presented deals with a male patient, aged 83 years, with diagnosis of panophthalmitis as atypical presentation of choroid melanoma. He assisted to the consultation of Ophthalmology Emergency with intense ocular pain in the right eye, accompanied by nausea and vomits. On examination, ocular hypertension was observed, that torpidly evolved during admission, with intraocular inflammation with extra-scleral extension. He had ocular ultrasound and computed axial tomography of orbits and was diagnosed with panophthalmitis secondary to choroid melanoma. The clinical findings of this atypical presentation form showed, whose definite treatment is enucleation. Histological diagnosis of necrotic choroid malignant melanoma was confirmed.
ABSTRACT
Objective To observe the effects of curcumin (Cur) on vasculogenic mimicry (VM) and PI3K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods Three-dimensional culture of OCM-1 cells was performed for the observation of VM formation and the influence of concentration gradient (0μmol · L-1,10 μmol · L-1,20 μmol · L-1,30 μmol · L-1,40 μmol · L-1) Cur on VM,and immunohis to chemistry staining was used to detect the expression of PI3K and EphA2 in OCM-1 cell line.Results Cur could markedly inhibit the formation of VM in choroid melanoma cell line OCM-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner,and the difference between groups with 0 μmol · L-1 Cur group was significant statistically (all P =0.000),but there was no significant difference between 30 μmol · L-1and40 μmol · L-1 group (EphA2,P=0.300;PI3K,P=0.188).Conclusion Cur can significantly inhibit VM formation in choroid melanoma cell line OCM-1,of which the mechanism may be related to the downregulation expression of EphA2 and PI3K,thus inhibiting PI3K signaling pathway.
ABSTRACT
Insulin-like growth factor 1 receptor (IGF-1 R),a member of the family of tyrosine protein kinase,mediates the activity of the IGF-1.IGF-1R can promote the proliferation and migration of cells and inhibit the apoptosis of cells.IGF-1R shows a high level expression and presents a close relationship with a variety of biological characteristics such as invasion and metastasis of choroid melanoma,and it influences the pathogenesis and progress of thyroid related ophthalmopathy,working together with thyroid stimulating hormone receptor (TSHR) through different signaling pathways.Also IGF-1 R,targeting vascular endothelial growth factor (VEGF),may promote the pathogenesis and development of new retinal vascular diseases through the insulin receptor substrate 1 (IRS-1).Other IGF-1R related biological effects were found in eye diseases,such as refractive error and cataract,ect.,hinting us that as a molecular targete,IGF-1R will become a new topic of gene therapy for eye diseases.